Purification, Characterization and Bactericidal Action of Lysozyme, Isolated from Bacillus subtillis BSN314: A Disintegrating Effect of Lysozyme on Gram-Positive and Gram-Negative Bacteria
In the present study, lysozyme was purified by the following multi-step methodology: salt (ammonium sulfate) precipitation, dialysis, and ultrafiltration.The lysozyme potential was measured by enzymatic activity after each purification step.However, after ultrafiltration, the resulting material was considered extra purified.It was concentrated in a